Responses of Cells to Flow in Vitro

Shigehiro HASHIMOTO, Fumihiko SATO, Kei OYA, Hiromichi FUJIE, Haruka IWATA and Yuma SAKATANI

ABSTRACT

The response of cells to the flow has been studied in vitro. The response of cells was examined in two types of flow channels: in a donut-shaped open channel, and in a parallelepiped flow channel. Variation was made on the material of the parallelepiped channel to study on adhesion of cells to the plates: glass and polydimethylsiloxane. Behavior of cells on the plate was observed under a flow of a medium with an inverted phase-contrast-microscope. The shear stress on the wall is calculated with an estimated parabolic distribution of the velocity between the parallel plates. The adhesion of cells was evaluated with the cumulated shear, which is a product of the shear stress and the exposure time. The experimental results show that cells are responsive to the flow, which governs orientation, exfoliation, and differentiation. The response depends on the kinds of cells: endothelial cells orient along the stream line, although myocytes orient perpendicular to the stream line. The adhesion depends on the combination between scaffold and cell: myocytes are more adhesive to glass than cartilage cells, and fibroblasts are more adhesive to polydimethylsiloxane than glass.

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